nir ingaas photon etc Search Results


alizé 1 7 ingaas camera  (Photon Etc)
86
Photon Etc alizé 1 7 ingaas camera
Alizé 1 7 Ingaas Camera, supplied by Photon Etc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/pmc12960835-420-18-11?v=Photon+Etc
Average 86 stars, based on 1 article reviews
alizé 1 7 ingaas camera - by Bioz Stars, 2026-06
86/100 stars
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nir ingaas photon etc  (Photon Etc)
86
Photon Etc nir ingaas photon etc
Nir Ingaas Photon Etc, supplied by Photon Etc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/pmc12994528-121-0-2?v=Photon+Etc
Average 86 stars, based on 1 article reviews
nir ingaas photon etc - by Bioz Stars, 2026-06
86/100 stars
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ingaas camera  (Raptor Photonics)
90
Raptor Photonics ingaas camera
a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of <t>an</t> <t>nIR-compatible</t> objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the <t>InGaAs</t> camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.
Ingaas Camera, supplied by Raptor Photonics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/pmc10505477-600-2-15?v=Raptor+Photonics
Average 90 stars, based on 1 article reviews
ingaas camera - by Bioz Stars, 2026-06
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ingaas detector for nir  (Hamamatsu)
90
Hamamatsu ingaas detector for nir
a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of <t>an</t> <t>nIR-compatible</t> objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the <t>InGaAs</t> camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.
Ingaas Detector For Nir, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/pmc07696914-76-16-20?v=Hamamatsu
Average 90 stars, based on 1 article reviews
ingaas detector for nir - by Bioz Stars, 2026-06
90/100 stars
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nir sensor basler aca-1600  (Basler)
90
Basler nir sensor basler aca-1600
a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of <t>an</t> <t>nIR-compatible</t> objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the <t>InGaAs</t> camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.
Nir Sensor Basler Aca 1600, supplied by Basler, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/10__1007_slash_s12567___021___00419___8-80-14-16?v=Basler
Average 90 stars, based on 1 article reviews
nir sensor basler aca-1600 - by Bioz Stars, 2026-06
90/100 stars
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ingaas nir photodetector r5509-73  (Hamamatsu)
90
Hamamatsu ingaas nir photodetector r5509-73
a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of <t>an</t> <t>nIR-compatible</t> objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the <t>InGaAs</t> camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.
Ingaas Nir Photodetector R5509 73, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/pmc12160971-116-9-13?v=Hamamatsu
Average 90 stars, based on 1 article reviews
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indium gallium arsenide photodiode  (OEC GmbH)
90
OEC GmbH indium gallium arsenide photodiode
a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of <t>an</t> <t>nIR-compatible</t> objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the <t>InGaAs</t> camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.
Indium Gallium Arsenide Photodiode, supplied by OEC GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/10__1063_slash_1__4936119-84-18-23?v=OEC+GmbH
Average 90 stars, based on 1 article reviews
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indium gallium arsenide (ingaas)  (Verlag GmbH)
90
Verlag GmbH indium gallium arsenide (ingaas)
a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of <t>an</t> <t>nIR-compatible</t> objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the <t>InGaAs</t> camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.
Indium Gallium Arsenide (Ingaas), supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/10__1002_slash_pssa__201600592-11-13-2?v=Verlag+GmbH
Average 90 stars, based on 1 article reviews
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90/100 stars
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indium gallium arsenide ingaas photodiode  (Thorlabs)
86
Thorlabs indium gallium arsenide ingaas photodiode
a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of <t>an</t> <t>nIR-compatible</t> objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the <t>InGaAs</t> camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.
Indium Gallium Arsenide Ingaas Photodiode, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/10__1109_slash_access__2025__3554139-73-5-11?v=Thorlabs
Average 86 stars, based on 1 article reviews
indium gallium arsenide ingaas photodiode - by Bioz Stars, 2026-06
86/100 stars
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indium gallium arsenide (ingaas) photodiode g9801-22  (Hamamatsu)
90
Hamamatsu indium gallium arsenide (ingaas) photodiode g9801-22
a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of <t>an</t> <t>nIR-compatible</t> objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the <t>InGaAs</t> camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.
Indium Gallium Arsenide (Ingaas) Photodiode G9801 22, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/10__1364_slash_ol__505390-48-25-31?v=Hamamatsu
Average 90 stars, based on 1 article reviews
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photodetector hca-s-200m-in  (FEMTO Messtechnik)
90
FEMTO Messtechnik photodetector hca-s-200m-in
a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of <t>an</t> <t>nIR-compatible</t> objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the <t>InGaAs</t> camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.
Photodetector Hca S 200m In, supplied by FEMTO Messtechnik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/10__1103_slash_physrevresearch__6__013005-53-1-5?v=FEMTO+Messtechnik
Average 90 stars, based on 1 article reviews
photodetector hca-s-200m-in - by Bioz Stars, 2026-06
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indium gallium arsenide (ingaas) photodiodes g6849  (Hamamatsu)
90
Hamamatsu indium gallium arsenide (ingaas) photodiodes g6849
a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of <t>an</t> <t>nIR-compatible</t> objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the <t>InGaAs</t> camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.
Indium Gallium Arsenide (Ingaas) Photodiodes G6849, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nir+ingaas+photon+etc/us11424827-394-9-24?v=Hamamatsu
Average 90 stars, based on 1 article reviews
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Image Search Results


a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of an nIR-compatible objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the InGaAs camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.

Journal: Nature protocols

Article Title: Near-infrared catecholamine nanosensors for high spatiotemporal dopamine imaging

doi: 10.1038/s41596-021-00530-4

Figure Lengend Snippet: a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of an nIR-compatible objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the InGaAs camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.

Article Snippet: Visible and nIR InGaAs camera (500–600 nm: 40% quantum efficiency (QE); 1,000–1,500 nm: >85% QE; Raptor Photonics, Ninox 640).

Techniques: Imaging, Fluorescence, Microscopy, Control, Labeling, Slice Preparation, Standard Deviation